O’Leary, Shannon J.Hollenbeck, ChristopherVega, Robert R.Gold, John R.Portnoy, David S.2022-02-212022-02-212018-02-23O’Leary, S.J., Hollenbeck, C.M., Vega, R.R., Gold, J.R. and Portnoy, D.S., 2018. Genetic mapping and comparative genomics to inform restoration enhancement and culture of southern flounder, Paralichthys lethostigma. BMC genomics, 19(1), pp.1-11.https://hdl.handle.net/1969.6/90169Southern flounder, Paralichthys lethostigma, historically support a substantial fishery along the Atlantic and Gulf coasts of the southern United States. Low year-class strengths over the past few years in the western Gulf of Mexico have raised concern that spawning stocks may be overfished. Current management of the resource includes releasing hatchery-raised juveniles to restock bays and estuaries; additionally, there is a growing interest in the potential for commercial aquaculture of the species. Currently, genomic resources for southern flounder do not exist. Here, we used two hatchery-reared families and double-digest, restriction-site-associated DNA (ddRAD) sequencing to create a reduced-representation genomic library consisting of several thousand single nucleotide polymorphisms (SNPs) located throughout the genome.en-USAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/linkage mapradseqaquaculturesyntenyArticlehttps://doi.org/10.1186/s12864-018-4541-0