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dc.contributor.advisorFelix O. Omoruyi
dc.contributor.advisorM. Jean Sparks
dc.contributor.authorRios, Lizette E.
dc.date.accessioned2017-02-21T16:45:06Z
dc.date.available2017-02-21T16:45:06Z
dc.date.issued2016-12
dc.identifier.urihttp://hdl.handle.net/1969.6/757
dc.descriptionA thesis Submitted in Partial Fulfillment of the Requirements for the Degree of MASTER OF SCIENCE IN BIOLOGY from Texas A&M University-Corpus Christi in Corpus Christi, Texas.en_US
dc.description.abstractThe aqueous preparation of Kalanchoe pinnata is traditionally used in the management of Type 2 diabetes mellitus (T2DM), but the effectiveness in curtailing the indices of T2DM is not clear. In this study, the efficacy of K. pinnata preparation in the treatment of T2DM animal models was investigated. Forty-eight Sprague-Dawley rats were divided into eight groups of six rats each as follows: Group 1: non-diabetic rats fed normal rat diet, group 2: non-diabetic rats fed high fat diet (HFD), group 3: Type 2 diabetic rats fed HFD, group 4: non-diabetic control group fed normal diet, group 5: non-diabetic control group fed HFD, group 6: diabetic control group, group 7: diabetic plus K. pinnata preparation, and group 8: diabetic plus Metformin. Groups 1 – 3 were euthanized after 21 days to generate baseline data. Thereafter, K. pinnata preparation or metformin was administered for 28 days. Animals were euthanized on day 28 after an overnight fast. Blood and organs were collected for various assays. Type 2 diabetic rats treated with K. pinnata preparation lost significant (P < 0.05) weight. Kalanchoe pinnata consumption resulted in decreased serum glucose. There was a significant (P < 0.05) increase in HDL and decrease in triglyceride levels. Alkaline phosphatase (ALP) and alanine amino transferase (ALT) activities, and blood urea nitrogen (BUN) and uric acid (UA) levels were significantly (P < 0.05) increased. Serum reduced glutathione (GSH) levels, superoxide dismutase (SOD) and hepatic pyruvate kinase (PK) activities were significantly (P < 0.05) elevated. Overall, results show that the consumption of K. pinnata preparation in Type 2 diabetic rats decreased body weight and serum glucose levels, as well as increased serum HDL and decreased triglyceride levels which may be beneficial in the effective management of the disease. Similarly, the observed increase in SOD activity and GSH levels in the diabetic rats may be protective against oxidative stress. The observed increase in hepatic PK activity may be indicative of improved glucose metabolism. However, the increase in serum ALP and ALT activities, and BUN and UA levels may be suggestive of hepatic and renal damage.en_US
dc.language.isoen_USen_US
dc.rightsThis material is made available for use in research, teaching, and private study, pursuant to U.S. Copyright law. The user assumes full responsibility for any use of the materials, including but not limited to, infringement of copyright and publication rights of reproduced materials. Any materials used should be fully credited with its source. All rights are reserved and retained regardless of current or future development or laws that may apply to fair use standards. Permission for publication of this material, in part or in full, must be secured with the author and/or publisher.en_US
dc.subjectcardiovascular diseaseen_US
dc.subjectdiabetesen_US
dc.subjecthyperglycermicen_US
dc.subjectKalanchoe pinnataen_US
dc.subjectoxidative stressen_US
dc.subjectstreptozotocinen_US
dc.titleKalanchoe pinnata preparation in the treatment of type 2 diabetes mellitusen_US
dc.typeTexten_US
thesis.degree.disciplineBiologyen_US
thesis.degree.grantorTexas A & M University--Corpus Christien_US
thesis.degree.levelMastersen_US
thesis.degree.nameMaster of Scienceen_US
dc.description.departmentLife Sciencesen_US
dc.description.collegeCollege of Science and Engineeringen_US
dc.type.genreThesisen_US


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