Bioactive ingredients in K. pinnata extract and the effects of combined K. pinnata and metformin preparations on antioxidant activities in diabetic and non-diabetic skeletal muscle cells

Simple item page
dc.contributor.authorRamon, Pedro
dc.contributor.authorBergmann, Daniela
dc.contributor.authorAbdulla, Hussain
dc.contributor.authorSparks, Jean
dc.contributor.authorOmoruyi, Felix
dc.creator.orcidhttps://orcid.org/0000-0002-5027-008Xen_US
dc.creator.orcidhttps://orcid.org/0000-0001-6926-8097en_US
dc.date.accessioned2023-03-27T15:15:18Z
dc.date.available2023-03-27T15:15:18Z
dc.date.issued2023-03-25
dc.description.abstractWith healthcare costs rising, many affected by ailments are turning to alternative medicine for treatment. More people are choosing to complement their pharmacological regimen with dietary supplements from natural products. In this study, the compound composition of Kalanchoe Pinnata (K. pinnata) and the effects of combined preparations of K. pinnata and metformin on antioxidant activity in human skeletal muscle myoblasts (HSMMs) and human diabetic skeletal muscle myoblasts (DHSMMs) were investigated. Ultraperformance liquid chromatography fusion orbitrap mass spectrometry (UPLC-OT-FTMS) identified biologically active flavanols in K. pinnata. The main compounds identified in locally grown K. pinnata were quercetin, kaempferol, apigenin, epigallocatechin gallate (EGCG), and avicularin. Antioxidant results indicated that a combinatorial preparation of K. pinnata with metformin may modulate antioxidant responses by increasing the enzymatic activity of superoxide dismutase and increasing levels of reduced glutathione. A combination of 50 µM and 150 µg/mL of metformin and K. pinnata, respectively, resulted in a significant increase in reduced glutathione levels in non-diabetic and diabetic human skeletal muscle myoblasts and H2O2-stress-induced human skeletal muscle myoblasts. Additionally, a K. pinnata treatment (400 µg/mL) alone significantly increased catalase (CAT) activity for non-diabetic and diabetic human skeletal muscle myoblasts and a H2O2-stress-induced human skeletal muscle myoblast cell line, while significantly lowering malondialdehyde (MDA) levels. However, the treatment options were more effective at promoting cell viability after 24 h versus 72 h and did not promote cell viability after 72 h in H2O2 -stress induced HSMM cells. These treatment options show promise for treating oxidative-stress-mediated pathophysiological complications associated with type II diabetes.en_US
dc.description.sponsorshipThis study was supported by the Grants-In-Aid of Chemistry Graduate Research Program funded by the Welch Foundation (2018–2019) to P.R. This work was supported by the National Science Foundation (OCE-1626494 to H.A.).en_US
dc.identifier.citationRamon, P.; Bergmann, D.; Abdulla, H.; Sparks, J.; Omoruyi, F. Bioactive Ingredients in K. pinnata Extract and the Effects of Combined K. pinnata and Metformin Preparations on Antioxidant Activities in Diabetic and Non-Diabetic Skeletal Muscle Cells. Int. J. Mol. Sci. 2023, 24, 6211. https://doi.org/10.3390/ ijms24076211en_US
dc.identifier.doihttps://doi.org/10.3390/ ijms24076211
dc.identifier.urihttps://hdl.handle.net/1969.6/95761
dc.language.isoen_USen_US
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectK. pinnataen_US
dc.subjectdiabetic skeletal muscle cellsen_US
dc.subjectoxidative stressen_US
dc.subjecttype II diabetes mellitusen_US
dc.titleBioactive ingredients in K. pinnata extract and the effects of combined K. pinnata and metformin preparations on antioxidant activities in diabetic and non-diabetic skeletal muscle cellsen_US
dc.typeArticleen_US

Files

Original bundle

Now showing 1 - 1 of 1
Thumbnail Image
Name:
Bioactive Ingredients in K. pinnata Extract and the Effects of Combined K. pinnata and Metformin Preparations on Antioxidant Activities in Diabetic and Non-Diabetic.pdf
Size:
2.9 MB
Format:
Adobe Portable Document Format
Description:
Download

License bundle

Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.72 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

CONHS Faculty Works