The effect of acute and chronic thermotherapy on type 2 diabetic skeletal muscle gene expression and inflammatory markers

Simple item page
dc.contributor.authorNewmire, Daniel
dc.contributor.authorOmoruyi, Felix
dc.contributor.authorSparks, Jean
dc.contributor.authorBachnak, Louay
dc.creator.orcidhttps://orcid.org/0000-0001-8666-0728en_US
dc.creator.orcidhttps://orcid.org/0000-0001-6926-8097en_US
dc.creator.orcidhttps://orcid.org/0000-0001-5176-3962en_US
dc.creator.orcidhttps://orcid.org/0000-0001-8666-0728
dc.creator.orcidhttps://orcid.org/0000-0001-6926-8097
dc.creator.orcidhttps://orcid.org/0000-0001-5176-3962
dc.date.accessioned2021-10-13T17:58:14Z
dc.date.available2021-10-13T17:58:14Z
dc.date.issued2021-09-20T23:00-06:00
dc.description.abstractBackground: Type 2 diabetes (T2D) is a chronic illness associated with resistance or defective insulin secretion. This study investigated the effects of thermotherapy on cell viability, gene expression, and inflammation in skeletal muscle cell lines. Methods: Healthy and T2D hu-man skeletal muscle cell lines (HSMM and D-HSMM, respectively) were subjected to acute or chronic thermo-therapy (AT or CT, respectively). The CT consisted of a 30-minute exposure to 40°C, three times a week for three weeks; the AT was a one-time exposure. Results: Significant de-crease in D-HSMM cell viability percentage followed the AT; however, no significant change occurred in CT. HSMM yielded the highest elevations of genes following the CT. In D-HSMM, both treatments yielded genes up-regulation. Both treatments significantly down-regulated IL-1β, IL-6, IL-10, and TNF-α in HSMM. AT significantly decreased IL-1β, IL-6, and upregulated IL-10 and TNF-α levels in D-HSMM, while CT yielded a reduction in IL-4, TNF-α, and an up-regulation of IL-6 and IL-10. Conclusion: Increase in gene expression indicates actin activity, and cellular responses, suggesting an increase in transcriptional regulation. The upregulation of IL-6 and IL-10 in D-HSMM negatively correlated with a decrease in TNF-α and IL-1β, indicating improved adverse inflammatory effects associated with the disease.en_US
dc.identifier.citation: Bachnak, L.; Sparks, J.; Newmire, D.E.; Gonzales, X.F.; Omoruyi, F.O. The Effect of Acute and Chronic Thermotherapy on Type 2 Diabetic Skeletal Muscle Gene Expression and Inflammatory Markers. Biomedicines 2021, 9, 1276. https://doi.org/10.3390/ biomedicines9091276en_US
dc.identifier.doihttps://doi.org/10.3390/ biomedicines9091276
dc.identifier.urihttps://hdl.handle.net/1969.6/89828
dc.language.isoen_USen_US
dc.publisherBiomedicines; Molecular and Translational Medicineen_US
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectthermotherapyen_US
dc.subjectType 2 Diabetesen_US
dc.subjectdiabetic skeletal muscle cellsen_US
dc.subjectheat treatment type II diabetesen_US
dc.titleThe effect of acute and chronic thermotherapy on type 2 diabetic skeletal muscle gene expression and inflammatory markersen_US
dc.typeArticleen_US

Files

Original bundle

Now showing 1 - 1 of 1
Thumbnail Image
Name:
Newmire_Daniel_Effect.pdf
Size:
4.62 MB
Format:
Adobe Portable Document Format
Description:
Download

License bundle

Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.72 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

COEHD Faculty Works