Preliminary assessment of microbial community structure of wind-tidal flats in the Laguna Madre, Texas, USA

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dc.contributor.authorHuang, I.-Shuo
dc.contributor.authorPinnell, Lee J.
dc.contributor.authorTurner, Jeffrey W.
dc.contributor.authorAbdulla, Hussain
dc.contributor.authorBoyd, Lauren
dc.contributor.authorLinton, Eric W.
dc.contributor.authorZimba, Paul V.
dc.creator.orcidhttps://orcid.org/0000-0002-6238-3313en_US
dc.creator.orcidhttps://orcid.org/0000-0002-9726-8649en_US
dc.creator.orcidhttps://orcid.org/0000-0001-7602-0137en_US
dc.creator.orcidhttps://orcid.org/0000-0003-0418-5244en_US
dc.creator.orcidhttps://orcid.org/0000-0001-6541-2055en_US
dc.creator.orcidhttps://orcid.org/0000-0002-6238-3313
dc.creator.orcidhttps://orcid.org/0000-0002-9726-8649
dc.creator.orcidhttps://orcid.org/0000-0001-7602-0137
dc.creator.orcidhttps://orcid.org/0000-0003-0418-5244
dc.creator.orcidhttps://orcid.org/0000-0001-6541-2055
dc.creator.orcidhttps://orcid.org/0000-0002-6238-3313
dc.creator.orcidhttps://orcid.org/0000-0002-9726-8649
dc.creator.orcidhttps://orcid.org/0000-0001-7602-0137
dc.creator.orcidhttps://orcid.org/0000-0003-0418-5244
dc.creator.orcidhttps://orcid.org/0000-0001-6541-2055https://orcid.org/0000-0002-6238-3313
dc.creator.orcidhttps://orcid.org/0000-0002-9726-8649
dc.creator.orcidhttps://orcid.org/0000-0001-7602-0137
dc.creator.orcidhttps://orcid.org/0000-0003-0418-5244
dc.creator.orcidhttps://orcid.org/0000-0001-6541-2055
dc.date.accessioned2021-10-21T17:02:09Z
dc.date.available2021-10-21T17:02:09Z
dc.date.issued2020-07-22
dc.description.abstractAside from two samples collected nearly 50 years ago, little is known about the microbial composition of wind tidal flats in the hypersaline Laguna Madre, Texas. These mats account for ~42% of the lagoon’s area. These microbial communities were sampled at four locations that historically had mats in the Laguna Madre, including Laguna Madre Field Station (LMFS), Nighthawk Bay (NH), and two locations in Kenedy Ranch (KRN and KRS). Amplicon sequencing of 16S genes determined the presence of 51 prokaryotic phyla dominated by Bacteroidota, Chloroflexi, Cyanobacteria, Desulfobacteria, Firmicutes, Halobacteria, and Proteobacteria. The microbial community structure of NH and KR is significantly different to LMFS, in which Bacteroidota and Proteobacteria were most abundant. Twenty-three cyanobacterial taxa were identified via genomic analysis, whereas 45 cyanobacterial taxa were identified using morphological analysis, containing large filamentous forms on the surface, and smaller, motile filamentous and coccoid forms in subsurface mat layers. Sample sites were dominated by species in Oscillatoriaceae (i.e., Lyngbya) and Coleofasciculaceae (i.e., Coleofasciculus). Most cyanobacterial sequences (~35%) could not be assigned to any established taxa at the family/genus level, given the limited knowledge of hypersaline cyanobacteria. A total of 73 cyanobacterial bioactive metabolites were identified using ultra performance liquid chromatography-Orbitrap MS analysis from these communities. Laguna Madre seems unique compared to other sabkhas in terms of its microbiology.en_US
dc.identifier.citationHuang, I., Pinnell, L.J., Turner, J.W., Abdulla, H., Boyd, L., Linton, E.W. and Zimba, P.V., 2020. Preliminary assessment of microbial community structure of wind-tidal flats in the Laguna Madre, Texas, USA. Biology, 9(8), p.183.en_US
dc.identifier.doihttps://doi.org/10.3390/biology9080183
dc.identifier.urihttps://hdl.handle.net/1969.6/89853
dc.language.isoen_USen_US
dc.publisherMDPIen_US
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjecthypersaline lagoonal wind-tidal microbial flatsen_US
dc.subjectlaguna madreen_US
dc.subjectcyanobacteriaen_US
dc.subjectmiseqen_US
dc.subjecttoxinsen_US
dc.subjectuplc-orbitrap msen_US
dc.titlePreliminary assessment of microbial community structure of wind-tidal flats in the Laguna Madre, Texas, USAen_US
dc.typeArticleen_US

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