Development of gene expression markers of acute heat-light stress in reef-building corals of the genus porites

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dc.contributor.authorKenkel, Carly
dc.contributor.authorAglyamova, Galina
dc.contributor.authorAlamaru, Ada
dc.contributor.authorBhagooli, Ranjeet
dc.contributor.authorCapper, Roxana
dc.contributor.authorCunning, Ross
dc.contributor.authordeVillers, Amanda
dc.contributor.authorHaslun, Joshua
dc.contributor.authorHédouin, Laetitia
dc.contributor.authorKeshavmurthy, Shashank
dc.contributor.authorKuehl, Kristin A.
dc.contributor.authorMahmoud, Huda
dc.contributor.authorMcGinty, Elizabeth S.
dc.contributor.authorMontoya Maya, Phanor Hernando
dc.contributor.authorPalmer, Caroline V.
dc.contributor.authorPantile, Raffaella
dc.contributor.authorSánchez, Juan A.
dc.contributor.authorSchils, Tom
dc.contributor.authorSilverstein, Rachel N.
dc.contributor.authorSquiers, Logan B.
dc.contributor.authorTang, Pei-Ciao
dc.contributor.authorGoulet, Tamar
dc.contributor.authorMatz, Mikhail
dc.contributor.authorKenkel, Carly
dc.contributor.authorAglyamova, Galina
dc.contributor.authorAlamaru, Ada
dc.contributor.authorBhagooli, Ranjeet
dc.contributor.authorCapper, Roxana
dc.contributor.authorCunning, Ross
dc.contributor.authordeVillers, Amanda
dc.contributor.authorHaslun, Joshua
dc.contributor.authorHédouin, Laetitia
dc.contributor.authorKeshavmurthy, Shashank
dc.contributor.authorKuehl, Kristin A.
dc.contributor.authorMahmoud, Huda
dc.contributor.authorMcGinty, Elizabeth S.
dc.contributor.authorMontoya Maya, Phanor Hernando
dc.contributor.authorPalmer, Caroline V.
dc.contributor.authorPantile, Raffaella
dc.contributor.authorSánchez, Juan A.
dc.contributor.authorSchils, Tom
dc.contributor.authorSilverstein, Rachel N.
dc.contributor.authorSquiers, Logan B.
dc.contributor.authorTang, Pei-Ciao
dc.contributor.authorGoulet, Tamar
dc.contributor.authorMatz, Mikhail
dc.creator.orcidhttps://orcid.org/0000-0003-1126-4311en_US
dc.creator.orcidhttps://orcid.org/0000-0002-5041-4047en_US
dc.creator.orcidhttps://orcid.org/0000-0002-1439-2186en_US
dc.creator.orcidhttps://orcid.org/0000-0001-7241-1181en_US
dc.creator.orcidhttps://orcid.org/0000-0002-0496-7943en_US
dc.creator.orcidhttps://orcid.org/0000-0002-6160-6175en_US
dc.creator.orcidhttps://orcid.org/0000-0002-1516-9082en_US
dc.creator.orcidhttps://orcid.org/0000-0002-0093-030Xen_US
dc.creator.orcidhttps://orcid.org/0000-0003-3883-6543en_US
dc.creator.orcidhttps://orcid.org/0000-0001-5453-9819en_US
dc.creator.orcidhttps://orcid.org/0000-0003-1126-4311
dc.creator.orcidhttps://orcid.org/0000-0002-5041-4047
dc.creator.orcidhttps://orcid.org/0000-0002-1439-2186
dc.creator.orcidhttps://orcid.org/0000-0001-7241-1181
dc.creator.orcidhttps://orcid.org/0000-0002-0496-7943
dc.creator.orcidhttps://orcid.org/0000-0002-6160-6175
dc.creator.orcidhttps://orcid.org/0000-0002-1516-9082
dc.creator.orcidhttps://orcid.org/0000-0002-0093-030X
dc.creator.orcidhttps://orcid.org/0000-0003-3883-6543
dc.creator.orcidhttps://orcid.org/0000-0001-5453-9819
dc.date.accessioned2022-04-01T19:18:59Z
dc.date.available2022-04-01T19:18:59Z
dc.date.issued2011-10-26
dc.date.issued2011-10-26
dc.description.abstractCoral reefs are declining worldwide due to increased incidence of climate-induced coral bleaching, which will have widespread biodiversity and economic impacts. A simple method to measure the sub-bleaching level of heat-light stress experienced by corals would greatly inform reef management practices by making it possible to assess the distribution of bleaching risks among individual reef sites. Gene expression analysis based on quantitative PCR (qPCR) can be used as a diagnostic tool to determine coral condition in situ. We evaluated the expression of 13 candidate genes during heat-light stress in a common Caribbean coral Porites astreoides, and observed strong and consistent changes in gene expression in two independent experiments. Furthermore, we found that the apparent return to baseline expression levels during a recovery phase was rapid, despite visible signs of colony bleaching. We show that the response to acute heat-light stress in P. astreoides can be monitored by measuring the difference in expression of only two genes: Hsp16 and actin. We demonstrate that this assay discriminates between corals sampled from two field sites experiencing different temperatures. We also show that the assay is applicable to an Indo-Pacific congener, P. lobata, and therefore could potentially be used to diagnose acute heat-light stress on coral reefs worldwide.en_US
dc.description.sponsorshipNational Science Foundation grant DEB-1054766 to MVM; Title V-Graduate programs/ELITE Graduate program: Event Travel Request program to LBS and JAH; Environmental Protection Agency Greater Research Opportunity Graduate Fellowship to KAK (MA-91697601); WIOMSA MARG III to PHM and RB; Aharon Katzir-Katchalsky Student Travel Fellowships and Anat Krauskopf Fund Travel Award to AA; NSF IOS-0747205 and funding from the University of Mississippi ORSP to TLG; Grants from both the National Science Council and Academia Sinica, Taiwan to KS; NSF GRF to RNS; Natural Environmental Research Council (NERC) studentship to CVP. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.en_US
dc.identifier.citationKenkel, C.D., Aglyamova, G., Alamaru, A., Bhagooli, R., Capper, R., Cunning, R., deVillers, A., Haslun, J.A., Hédouin, L., Keshavmurthy, S. and Kuehl, K.A., 2011. Development of gene expression markers of acute heat-light stress in reef-building corals of the genus Porites. PloS one, 6(10), p.e26914.en_US
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0026914
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0026914
dc.identifier.urihttps://hdl.handle.net/1969.6/90394
dc.identifier.urihttps://hdl.handle.net/1969.6/90394
dc.language.isoen_USen_US
dc.publisherPLOS ONEen_US
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectgeneen_US
dc.subjectreef-buildingen_US
dc.subjectacute light stressen_US
dc.titleDevelopment of gene expression markers of acute heat-light stress in reef-building corals of the genus poritesen_US
dc.typeArticleen_US

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