A multiplex analysis of potentially toxic cyanobacteria in Lake Winnipeg during the 2013 bloom season

Simple item page
dc.contributor.authorMcKindles, Katelyn M.
dc.contributor.authorZimba, Paul V.
dc.contributor.authorChiu, Alexander S.
dc.contributor.authorWatson, Susan B.
dc.contributor.authorGutierrez, Danielle B.
dc.contributor.authorWestrick, Judy
dc.contributor.authorKling, Hedy
dc.contributor.authorDavis, Timothy W.
dc.creator.orcidhttps://orcid.org/0000-0001-6541-2055en_US
dc.creator.orcidhttps://orcid.org/0000-0001-6541-2055
dc.creator.orcidhttps://orcid.org/0000-0001-6541-2055https://orcid.org/0000-0001-6541-2055
dc.date.accessioned2021-10-27T15:18:27Z
dc.date.available2021-10-27T15:18:27Z
dc.date.issued2019-10-11
dc.description.abstractLake Winnipeg (Manitoba, Canada), the world’s 12th largest lake by area, is host to yearly cyanobacterial harmful algal blooms (cHABs) dominated by Aphanizomenon and Dolichospermum. cHABs in Lake Winnipeg are primarily a result of eutrophication but may be exacerbated by the recent introduction of dreissenid mussels. Through multiple methods to monitor the potential for toxin production in Lake Winnipeg in conjunction with environmental measures, this study defined the baseline composition of a Lake Winnipeg cHAB to measure potential changes because of dreissenid colonization. Surface water samples were collected in 2013 from 23 sites during summer and from 18 sites in fall. Genetic data and mass spectrometry cyanotoxin profiles identified microcystins (MC) as the most abundant cyanotoxin across all stations, with MC concentrations highest in the north basin. In the fall, mcyA genes were sequenced to determine which species had the potential to produce MCs, and 12 of the 18 sites were a mix of both Planktothrix and Microcystis. Current blooms in Lake Winnipeg produce low levels of MCs, but the capacity to produce cyanotoxins is widespread across both basins. If dreissenid mussels continue to colonize Lake Winnipeg, a shift in physicochemical properties of the lake because of faster water column clearance rates may yield more toxic blooms potentially dominated by microcystin producers.en_US
dc.identifier.citationMcKindles, K.M., Zimba, P.V., Chiu, A.S., Watson, S.B., Gutierrez, D.B., Westrick, J., Kling, H. and Davis, T.W., 2019. A multiplex analysis of potentially toxic cyanobacteria in Lake Winnipeg during the 2013 bloom season. Toxins, 11(10), p.587.en_US
dc.identifier.doihttps://doi.org/10.3390/toxins11100587
dc.identifier.urihttps://hdl.handle.net/1969.6/89866
dc.language.isoen_USen_US
dc.publisherMDPIen_US
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectcyanobacteriaen_US
dc.subjectcylindrospermopsinen_US
dc.subjectmicrocystinen_US
dc.subjectmultiplex qpcren_US
dc.subjectlake winnipegen_US
dc.subjectsaxitoxinen_US
dc.titleA multiplex analysis of potentially toxic cyanobacteria in Lake Winnipeg during the 2013 bloom seasonen_US
dc.typeArticleen_US

Files

Original bundle

Now showing 1 - 1 of 1
Thumbnail Image
Name:
McKindles_Katelyn_Toxins.pdf
Size:
3.41 MB
Format:
Adobe Portable Document Format
Description:
Article
Download

License bundle

Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.72 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

COS Faculty Works