Shotgun metagenomics reveals the benthic microbial community response to plastic and bioplastic in a coastal marine environment

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dc.contributor.authorTurner, Jeffrey W.
dc.contributor.authorPinnell, Lee J.
dc.date.accessioned2022-02-02T18:53:23Z
dc.date.available2022-02-02T18:53:23Z
dc.date.issued2019-06-07
dc.description.abstractPlastic is incredibly abundant in marine environments but little is known about its effects on benthic microbiota and biogeochemical cycling. This study reports the shotgun metagenomic sequencing of biofilms fouling plastic and bioplastic microcosms staged at the sediment–water interface of a coastal lagoon. Community composition analysis revealed that plastic biofilms were indistinguishable in comparison to a ceramic biofilm control. By contrast, bioplastic biofilms were distinct and dominated by sulfate-reducing microorganisms (SRM). Analysis of bioplastic gene pools revealed the enrichment of esterases, depolymerases, adenylyl sulfate reductases (aprBA), and dissimilatory sulfite reductases (dsrAB). The nearly 20-fold enrichment of a phylogenetically diverse polyhydroxybutyrate (PHB) depolymerase suggests this gene was distributed across a mixed microbial assemblage. The metagenomic reconstruction of genomes identified novel species of Desulfovibrio, Desulfobacteraceae, and Desulfobulbaceae among the abundant SRM, and these genomes contained genes integral to both bioplastic degradation and sulfate reduction. Findings indicate that bioplastic promoted a rapid and significant shift in benthic microbial diversity and gene pools, selecting for microbes that participate in bioplastic degradation and sulfate reduction. If plastic pollution is traded for bioplastic pollution and sedimentary inputs are large, the microbial response could unintentionally affect benthic biogeochemical activities through the stimulation of sulfate reducers.en_US
dc.identifier.citationPinnell, L.J. and Turner, J.W., 2019. Shotgun metagenomics reveals the benthic microbial community response to plastic and bioplastic in a coastal marine environment. Frontiers in microbiology, 10, p.1252.en_US
dc.identifier.doihttps://doi.org/10.3389/fmicb.2019.01252
dc.identifier.urihttps://hdl.handle.net/1969.6/90137
dc.language.isoen_USen_US
dc.publisherFrontiers in Microbiologyen_US
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectplasticen_US
dc.subjectbioplasticen_US
dc.subjectbiodegradationen_US
dc.subjectsulfur cyclingen_US
dc.subjectcoastal sedimentsen_US
dc.subjectmetagenomicsen_US
dc.titleShotgun metagenomics reveals the benthic microbial community response to plastic and bioplastic in a coastal marine environmenten_US
dc.typeArticleen_US

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