Development of a green fluorescent protein-based laboratory curriculum
Date
2005-01
Authors
Larkin, Patrick
ORCID
Journal Title
Journal ISSN
Volume Title
Publisher
IUMBM Journals
Abstract
A laboratory curriculum has been designed for an undergraduate biochemistry course that focuses on the investigation of the green fluorescent protein (GFP). The sequence of procedures extends from analysis of the DNA sequence through PCR amplification, recombinant plasmid DNA synthesis, bacterial transformation, expression, isolation, and characterization of the protein by SDS-PAGE. A survey of participants found that the majority of them were performing most of these procedures for the first time and that participants found the exercises enjoyable and considered them a significant aid to their understanding of biochemistry, cell biology, and molecular genetics.
Description
Keywords
green fluorescent protein, green fluorescent, biochemistry, cell biology, molecular genetics, curriculum, exercise
Sponsorship
Rights:
Attribution-NonCommercial 4.0 International
Citation
Larkin, P.D. and Hartberg, Y., 2005. Development of a green fluorescent protein‐based laboratory curriculum. Biochemistry and Molecular Biology Education, 33(1), pp.41-45.