Quantitative autofluorescence and cell density maps of the human retinal pigment epithelium

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dc.contributor.authorAch, Thomas
dc.contributor.authorHuisingh, Carrie
dc.contributor.authorMcGwin, Gerald
dc.contributor.authorMessinger, Jeffrey
dc.contributor.authorZhang, Tianjiao
dc.contributor.authorBentley, Mark
dc.contributor.authorGutierrez, Danielle B.
dc.contributor.authorAblonczy, Zsolt
dc.contributor.authorSmith, R. Theodore
dc.contributor.authorSloan, Kenneth R.
dc.contributor.authorCurcio, Christine A.
dc.creator.orcidhttp://orcid.org/0000-0002-7010-2024en_US
dc.creator.orcidhttp://orcid.org/0000-0001-9592-1133en_US
dc.creator.orcidhttp://orcid.org/0000-0002-5975-3862en_US
dc.creator.orcidhttp://orcid.org/0000-0002-4780-1767en_US
dc.creator.orcidhttp://orcid.org/0000-0001-6355-2134en_US
dc.creator.orcidhttp://orcid.org/0000-0003-4441-8022en_US
dc.creator.orcidhttp://orcid.org/0000-0003-0182-1962en_US
dc.creator.orcidhttps://orcid.org/0000-0002-7010-2024
dc.creator.orcidhttps://orcid.org/0000-0001-9592-1133
dc.creator.orcidhttps://orcid.org/0000-0002-5975-3862
dc.creator.orcidhttps://orcid.org/0000-0002-4780-1767
dc.creator.orcidhttps://orcid.org/0000-0001-6355-2134
dc.creator.orcidhttps://orcid.org/0000-0003-4441-8022
dc.creator.orcidhttps://orcid.org/0000-0003-0182-1962
dc.creator.orcidhttps://orcid.org/0000-0002-7010-2024
dc.creator.orcidhttps://orcid.org/0000-0001-9592-1133
dc.creator.orcidhttps://orcid.org/0000-0002-5975-3862
dc.creator.orcidhttps://orcid.org/0000-0002-4780-1767
dc.creator.orcidhttps://orcid.org/0000-0001-6355-2134
dc.creator.orcidhttps://orcid.org/0000-0003-4441-8022
dc.creator.orcidhttps://orcid.org/0000-0003-0182-1962http://orcid.org/0000-0002-7010-2024
dc.creator.orcidhttp://orcid.org/0000-0001-9592-1133
dc.creator.orcidhttp://orcid.org/0000-0002-5975-3862
dc.creator.orcidhttp://orcid.org/0000-0002-4780-1767
dc.creator.orcidhttp://orcid.org/0000-0001-6355-2134
dc.creator.orcidhttp://orcid.org/0000-0003-4441-8022
dc.creator.orcidhttp://orcid.org/0000-0003-0182-1962
dc.date.accessioned2022-03-15T14:07:50Z
dc.date.available2022-03-15T14:07:50Z
dc.date.issued2014-08
dc.description.abstractPurpose.: Lipofuscin (LF) accumulation within RPE cells is considered pathogenic in AMD. To test whether LF contributes to RPE cell loss in aging and to provide a cellular basis for fundus autofluorescence (AF) we created maps of human RPE cell number and histologic AF. Methods.: Retinal pigment epithelium–Bruch's membrane flat mounts were prepared from 20 donor eyes (10 ≤ 51 and 10 > 80 years; postmortem: ≤4.2 hours; no retinal pathologies), preserving foveal position. Phalloidin-binding RPE cytoskeleton and LF-AF (488-nm excitation) were imaged at up to 90 predefined positions. Maps were assembled from 83,330 cells in 1470 locations. From Voronoi regions representing each cell, the number of neighbors, cell area, and total AF intensity normalized to an AF standard was determined. Results.: Highly variable between individuals, RPE-AF increases significantly with age. A perifoveal ring of high AF mirrors rod photoreceptor topography and fundus-AF. Retinal pigment epithelium cell density peaks at the fovea, independent of age, yet no net RPE cell loss is detectable. The RPE monolayer undergoes considerable lifelong re-modeling. The relationship of cell size and AF, a surrogate for LF concentration, is orderly and linear in both groups. Autofluorescence topography differs distinctly from the topography of age-related rod loss. Conclusions.: Digital maps of quantitative AF, cell density, and packing geometry provide metrics for cellular-resolution clinical imaging and model systems. The uncoupling of RPE LF content, cell number, and photoreceptor topography in aging challenges LF's role in AMD.en_US
dc.identifier.citationAch, T., Huisingh, C., McGwin, G., Messinger, J.D., Zhang, T., Bentley, M.J., Gutierrez, D.B., Ablonczy, Z., Smith, R.T., Sloan, K.R. and Curcio, C.A., 2014. Quantitative autofluorescence and cell density maps of the human retinal pigment epithelium. Investigative ophthalmology & visual science, 55(8), pp.4832-4841.en_US
dc.identifier.doihttps://doi.org/10.1167/iovs.14-14802
dc.identifier.urihttps://hdl.handle.net/1969.6/90267
dc.language.isoen_USen_US
dc.publisherAssociation for Research in Vision and Ophthalmologyen_US
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectretinalen_US
dc.subjectretinal pigment epitheliumen_US
dc.subjectautofluorescenceen_US
dc.subjectphotoreceptoren_US
dc.subjectcytoskeletonen_US
dc.titleQuantitative autofluorescence and cell density maps of the human retinal pigment epitheliumen_US
dc.typeArticleen_US

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